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Social media platforms are accused repeatedly of creating environments in which women are bullied and harassed. We argue that online aggression toward women aims to reinforce traditional feminine norms and stereotypes. In a mixed methods study, we find that this type of aggression on Twitter is common and extensive and that it can spread far beyond the original target. We locate over 2.9 million tweets in one week that contain instances of gendered insults (e.g., “bitch,” “cunt,” “slut,” or “whore”)—averaging 419,000 sexist slurs per day. The vast majority of these tweets are negative in sentiment. We analyze the social networks of the conversations that ensue in several cases and demonstrate how the use of “replies,” “retweets,” and “likes” can further victimize a target. Additionally, we develop a sentiment classifier that we use in a regression analysis to compare the negativity of sexist messages. We find that words in a message that reinforce feminine stereotypes inflate the negative sentiment of tweets to a significant and sizeable degree. These terms include those insulting someone’s appearance (e.g., “ugly”), intellect (e.g., “stupid”), sexual experience (e.g., “promiscuous”), mental stability (e.g., “crazy”), and age (“old”). Messages enforcing beauty norms tend to be particularly negative. In sum, hostile, sexist tweets are strategic in nature. They aim to promote traditional, cultural beliefs about femininity, such as beauty ideals, and they shame victims by accusing them of falling short of these standards. Harassment on social media constitutes an everyday, routine occurrence, with researchers finding 9,764,583 messages referencing bullying on Twitter over the span of two years (Bellmore et al. 2015). In other words, Twitter users post over 13,000 bullying-related messages on a daily basis. Forms of online aggression also carry with them serious, negative consequences. Repeated research documents that bullying victims suffer from a host of deleterious outcomes, such as low self-esteem (Hinduja and Patchin 2010), emotional and psychological distress (Ybarra et al. 2006), and negative emotions (Faris and Felmlee 2014; Juvonen and Gross 2008). Compared to those who have not been attacked, victims also tend to report more incidents of suicide ideation and attempted suicide (Hinduja and Patchin 2010). Several studies document that the targets of cyberbullying are disproportionately women (Backe et al. 2018; Felmlee and Faris 2016; Hinduja and Patchin 2010; Pew Research Center 2017), although there are exceptions depending on definitions and venues. Yet, we know little about the content or pattern of cyber aggression directed toward women in online forums. The purpose of the present research, therefore, is to examine in detail the practice of aggressive messaging that targets women and femininity within the social media venue of Twitter. Using both qualitative and quantitative analyses, we investigate the role of gender norm regulation in these patterns of cyber aggression.